SIFT predicts whether an amino acid substitution affects protein function based on sequence homology and the physical properties of amino acids. SIFT can be applied to naturally occurring nonsynonymous polymorphisms and laboratory-induced missense mutations.

Other prediction tools for amino acid substitutions: PolyPhen , MAPP , SNPs3D, PMut


Code & Data
SIFT for in-house use (version 3.0, released March 21, 2008):
Copyright code & exe (Sun, Linux) Report bugs

Prediction on human SNPs (Genome Research 12: 436-446 pdf)
Initial training/test sets for SIFT: LacI, Lysozyme, HIV protease

Other prediction tools for amino acid substitutions: PolyPhen , MAPP , SNPs3D, PMut



Literature
Our review "Predicting the Effects of Amino Acid Substitutions on Protein Function" in Annual Review of Genomics and Human Genetics:
Chapter
Supplementary Table 1

Referencing SIFT
Predicting Deleterious Amino Acid Substitutions, Genome Res. 2001 May; 11(5): 863.874. (Link),
Accounting for Human Polymorphisms Predicted to Affect Protein Function, Genome Res. 2002 December; 436-446 (Link)
SIFT: predicting amino acid changes that affect protein function, Nucleic Acids Research, 2003, Vol. 31, No. 13 3812-3814 (Link)

SIFT help page
SIFT Toolbox


submit a list of genomic coordinates New!



submit a list of multiple proteins and their substitutions (limit to 1000 entries please!) New!



submit dbSNP IDs (SNPs from multiple proteins, 2 minutes)



submit a GI Number (2 minutes).



submit a protein sequence (10-15 minutes)



submit a query sequence along with related sequences (< 1 minute)



submit alignment of your query sequence with related sequences (< 1 minute)




Page last modified November 2008
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Supported by the National Human Genome Research Institute (R01 HG004701-01 )