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SIFT Human DB (release 63)

SIFT dbSNP DB (build 132)

Related links

Human genome assembly GRCh37

Ensembl annotation release 63

NCBI dbSNP Build 132

NCBI BLink

Updates

Aug 2011: SIFT Human DB updated to support GRCh37 Ensembl release 63

Apr 2011: SIFT dbSNP DB updated to support NCBI dbSNP build 132

Referencing SIFT

Kumar P, Henikoff S, Ng PC. Predicting the effects of coding non-synonymous variants on protein function using the SIFT algorithm. Nat Protoc. 2009;4(7):1073-81. PubMed PDF

Ng PC, Henikoff S. Predicting the Effects of Amino Acid Substitutions on Protein Function Annu Rev Genomics Hum Genet. 2006;7:61-80. PubMed PDF Supp

Ng PC, Henikoff S. SIFT: predicting amino acid changes that affect protein function. Nucleic Acids Res. 2003 Jul 1;31(13):3812-4. PubMed

Ng PC, Henikoff S. Accounting for Human Polymorphisms Predicted to Affect Protein Function. Genome Res. 2002 Mar;12(3):436-46. PubMed Data

Ng PC, Henikoff S. Predicting Deleterious Amino Acid Substitutions. Genome Res. 2001 May;11(5):863-74. PubMed

SIFT predicts whether an amino acid substitution affects protein function. SIFT prediction is based on the degree of conservation of amino acid residues in sequence alignments derived from closely related sequences, collected through PSI-BLAST. SIFT can be applied to naturally occurring nonsynonymous polymorphisms or laboratory-induced missense mutations.

SIFT Human Genome DB	Tool Description (GRCh37 assembly Ensembl 63)
SIFT Human SNPs	Get SIFT predictions for nonsynonymous SNPs (Sample format)
	Other human genome tools: Restrict to Coding Variants (Sample format) Classify Human indels (Sample format)
SIFT Human Protein DB	Tool Description (Ensembl 63)
SIFT Human Protein NEW	Get SIFT predictions for nonsynonymous AA substitutions (Ensembl ENSP ID)
SIFT dbSNP DB	Tool Description (dbSNP Build 132)
SIFT dbSNP rs IDs	Get SIFT predictions for dbSNP SNPs including non-human species (NCBI rs ID)
SIFT dbSNP Protein	Get SIFT predictions for dbSNP proteins including non-human species (RefSeq ID or GI number)
SIFT Single Protein Tools	Tool Description
SIFT BLink	Run SIFT analysis on single protein using precomputed BLAST from NCBI BLink (RefSeq ID or GI number)
SIFT Sequence	Run SIFT analysis on single protein through a PSI-BLAST search (fasta)
SIFT Related Sequences	Run SIFT analysis on protein query and a group of related sequences (multi-fasta)
SIFT Aligned Sequences	Run SIFT analysis on protein query already in multi-sequence alignments (MSA)